Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFIB

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
cervix
tissue
cervix
cell line
HeLa
cell type
human cervical cancer
chip antibody
anti-NFIB (Bethyl #A303-566A)

Sequenced DNA Library

library_name
GSM6781512
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using Biogenode Bioruptor 300. ChIP-seq libraries were prepared using the NEB Ultra DNA library prep kit. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler.

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg38

Number of total reads
26475277
Reads aligned (%)
93.6
Duplicates removed (%)
6.3
Number of peaks
3482 (qval < 1E-05)

hg19

Number of total reads
26475277
Reads aligned (%)
92.9
Duplicates removed (%)
7.6
Number of peaks
3514 (qval < 1E-05)

Base call quality data from DBCLS SRA