GSM6781512: HeLa NFIB be rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NFIB
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
cervix
tissue
cervix
cell line
HeLa
cell type
human cervical cancer
chip antibody
anti-NFIB (Bethyl #A303-566A)
Sequenced DNA Library
library_name
GSM6781512
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using Biogenode Bioruptor 300. ChIP-seq libraries were prepared using the NEB Ultra DNA library prep kit. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler.